Overview

  • Product name
  • Description
    Rabbit polyclonal to BNP
  • Tested applications
    Suitable for: IHC-P, ELISA, RIA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) conjugated to bovine thyroglobulin.

Properties

Applications

Our Abpromise guarantee covers the use of ab19646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • BNP(1-28) antibody
    • BNP(1-29) antibody
    • BNP(1-30) antibody
    • BNP(1-32) antibody
    • BNP(2-31) antibody
    • BNP(3-29) antibody
    • BNP(3-30) antibody
    • BNP(3-32) antibody
    • BNP(4-27) antibody
    • BNP(4-29) antibody
    • BNP(4-30) antibody
    • BNP(4-31) antibody
    • BNP(4-32) antibody
    • BNP(5-29) antibody
    • BNP(5-31) antibody
    • BNP(5-32) antibody
    • Brain natriuretic peptide 32 antibody
    • Brain type natriuretic peptide antibody
    • Gamma brain natriuretic peptide antibody
    • Natriuretic peptide B antibody
    • Natriuretic peptide precursor B antibody
    • natriuretic peptide, brain type antibody
    • Natriuretic peptides B antibody
    • natriuretic protein antibody
    • NPPB antibody
    see all

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iCell® Cardiomyocytes)
Permeabilization
No
Specification
iCell® Cardiomyocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Aug 31 2015

Application
Flow Cytometry
Fixation
Acetone
Permeabilization
No
Sample
Human Cell (iCell­ Cardiomyocytes)
Specification
iCell­ Cardiomyocytes
Gating Strategy
negative control cells
Preparation
Cell harvesting/tissue preparation method: 1 mM EDTA/1 mM EGTA in PBS (calcium- and magnesium-free)
Sample buffer: DPBS
Username

Abcam user community

Verified customer

Submitted Feb 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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