Anti-BNP antibody [EPR3735] (ab92500)

Overview

  • Product nameAnti-BNP antibody [EPR3735]
    See all BNP primary antibodies
  • Description
    Rabbit monoclonal [EPR3735] to BNP
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human BNP.

  • Positive control
    • Human plasma and fetal heart lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/2000 - 1/20000. Detects a band of approximately 30 kDa (predicted molecular weight: 15 kDa).

    Is unsuitable for IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBrain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • BNP(1-28) antibody
      • BNP(1-29) antibody
      • BNP(1-30) antibody
      • BNP(1-32) antibody
      • BNP(2-31) antibody
      • BNP(3-29) antibody
      • BNP(3-30) antibody
      • BNP(3-32) antibody
      • BNP(4-27) antibody
      • BNP(4-29) antibody
      • BNP(4-30) antibody
      • BNP(4-31) antibody
      • BNP(4-32) antibody
      • BNP(5-29) antibody
      • BNP(5-31) antibody
      • BNP(5-32) antibody
      • Brain natriuretic peptide 32 antibody
      • Brain type natriuretic peptide antibody
      • Gamma brain natriuretic peptide antibody
      • Natriuretic peptide B antibody
      • Natriuretic peptide precursor B antibody
      • natriuretic peptide, brain type antibody
      • Natriuretic peptides B antibody
      • natriuretic protein antibody
      • NPPB antibody
      see all

    Anti-BNP antibody [EPR3735] images

    • All lanes : Anti-BNP antibody [EPR3735] (ab92500) at 1/2000 dilution

      Lane 1 : Human plasma
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 15 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    References for Anti-BNP antibody [EPR3735] (ab92500)

    ab92500 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply and your interest in Abcam antibodies.

    For a quotation, please contact our Australian distributor Sapphire Bioscience Pty Ltd.
    Their contact details are :
    126 Cope Street
    Waterloo - NSW
    2017
    ...

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    Thank you for contacting us.

    proBNP:

    As shown on the attached file (ab92500 v proBNP.pdf), ab92500seems to be suitable for your work as it would bind to the proBNP at a different region than the Mouse antibody that binds the region ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"