Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab105181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 15 kDa).

Target

  • FunctionCardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
  • Tissue specificityBrain and also in atria, but at much lower levels than ANP.
  • Sequence similaritiesBelongs to the natriuretic peptide family.
  • Post-translational
    modifications
    The brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro).
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANFB_HUMAN antibody
    • BNP antibody
    • BNP(1-32) antibody
    • BNP(5-29) antibody
    • BNP-32 antibody
    • Brain type natriuretic peptide antibody
    • Gamma brain natriuretic peptide antibody
    • Gamma-brain natriuretic peptide antibody
    • Natriuretic peptide B antibody
    • Natriuretic peptide precursor B antibody
    • Natriuretic protein antibody
    • nppb antibody
    see all

Anti-BNP/ProBNP antibody images

  • All lanes : Anti-BNP/ProBNP antibody (ab105181) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human heart tissue lysate - total protein (ab29431) with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 108 kDa,73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes

References for Anti-BNP/ProBNP antibody (ab105181)

ab105181 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"