The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
Use a concentration of 0.01 - 0.1 µg/ml.
Use a concentration of 2 - 5 µg/ml.
FunctionCardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.
Tissue specificityBrain and also in atria, but at much lower levels than ANP.
Sequence similaritiesBelongs to the natriuretic peptide family.
Post-translational modificationsThe brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro).