The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 27 kDa).
FunctionTranscriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Tissue specificityB-cell specific.
Involvement in diseaseNote=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
Sequence similaritiesBelongs to the POU2AF1 family.
Post-translational modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Lane 1 : Anti-BOB1 antibody (ab118389) at 0.5 µg/ml Lane 2 : Anti-BOB1 antibody (ab118389) at 1 mg/ml Lane 3 : Anti-DYKDDDDK Tag antibody at 1/3000 dilution
Lane 1 : HEK293 cell lyaste, transfected with full-length Human BOB1 (myc and DYKDDDDK tagged) Lane 2 : HEK293 cell lyaste, mock transfected Lane 3 : HEK293 cell lyaste, transfected with full-length Human BOB1 (myc and DYKDDDDK tagged)
Lysates/proteins at 10 µg per lane.
Predicted band size : 27 kDa
References for Anti-BOB1 antibody (ab118389)
has not yet been referenced specifically in any publications.
Publishing research using ab118389? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"