Anti-BOB1 antibody (ab3426)
Key features and details
- Goat polyclonal to BOB1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
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Overview
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Product name
Anti-BOB1 antibody
See all BOB1 primary antibodies -
Description
Goat polyclonal to BOB1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human -
Immunogen
Synthetic peptide: DAYALNHTLSVEGF, corresponding to C terminal amino acids 243-256 of Human BOB1.
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Positive control
- Mouse spleen lysate.
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General notes
Principal Names – POU2AF1; BOB1; OBF1; OCAB; OBF-1; POU domain, class 2, associating factor 1. Official Gene Symbol - POU2AF1. GenBank Accession Number – NP_006226. LocusLink ID - 5450 (human); 18985 (mouse). Gene Ontology terms - transcription cofactor activity; transcription co-activator activity; transcription activating factor; humoral immune response; transcription from Pol II promoter.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Constituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride -
Concentration information loading...
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Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
A 1 hour primary incubation is recommended for this product. Detects a band of approximately 30 kDa in Mouse Spleen lysate. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). A 1 hour primary incubation is recommended for this product. Detects a band of approximately 30 kDa in Mouse Spleen lysate. |
Target
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Function
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. -
Tissue specificity
B-cell specific. -
Involvement in disease
Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. -
Sequence similarities
Belongs to the POU2AF1 family. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5450 Human
- Entrez Gene: 18985 Mouse
- Omim: 601206 Human
- SwissProt: Q16633 Human
- SwissProt: Q64693 Mouse
- Unigene: 654525 Human
- Unigene: 897 Mouse
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Alternative names
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
Images
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ab3426 at a 1
µ g/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30µ g total protein per lane, 1 hour incubation) by Western blot (ECL). ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL).
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab3426 has been referenced in 2 publications.
- Russo MV et al. Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury. Nat Immunol 19:442-452 (2018). PubMed: 29662169
- Joza S et al. Loss of semaphorin-neuropilin-1 signaling causes dysmorphic vascularization reminiscent of alveolar capillary dysplasia. Am J Pathol 181:2003-17 (2012). PubMed: 23063659