Recombinant Anti-BOB1 antibody [EPR3299] (ab92315)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3299] to BOB1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BOB1 antibody [EPR3299]
See all BOB1 primary antibodies -
Description
Rabbit monoclonal [EPR3299] to BOB1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BOB1 aa 50-150. The exact sequence is proprietary.
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Positive control
- Ramos, Daudi, NAMALWA or Raji nuclear lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3299 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 28 kDa. |
Target
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Function
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. -
Tissue specificity
B-cell specific. -
Involvement in disease
Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. -
Sequence similarities
Belongs to the POU2AF1 family. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5450 Human
- Omim: 601206 Human
- SwissProt: Q16633 Human
- Unigene: 654525 Human
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Alternative names
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
Images
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All lanes : Anti-BOB1 antibody [EPR3299] (ab92315) at 1/2000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : NAMALWA cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab92315 has been referenced in 1 publication.
- Chu CS et al. Unique Immune Cell Coactivators Specify Locus Control Region Function and Cell Stage. Mol Cell 80:845-861.e10 (2020). PubMed: 33232656