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Overview

  • Product nameAnti-BOB1 antibody [SP92]
    See all BOB1 primary antibodies
  • Description
    Rabbit monoclonal [SP92] to BOB1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to C-terminus of Human BOB1.

  • Positive control
    • Human Tonsil
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab99891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P 1/100. Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • FunctionTranscriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
  • Tissue specificityB-cell specific.
  • Involvement in diseaseNote=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
  • Sequence similaritiesBelongs to the POU2AF1 family.
  • Post-translational
    modifications    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • B cell Oct binding protein 1 antibody
      • B cell specific coactivator OBF 1 antibody
      • B cell specific coactivator OBF1 antibody
      • B-cell-specific coactivator OBF-1 antibody
      • BOB 1 antibody
      • BOB-1 antibody
      • OBF 1 antibody
      • OBF1 antibody
      • OBF1_HUMAN antibody
      • OCA B antibody
      • OCA-B antibody
      • OCAB antibody
      • OCT binding factor 1 antibody
      • OCT-binding factor 1 antibody
      • POU class 2 associating factor 1 antibody
      • POU domain class 2 associating factor 1 antibody
      • POU domain class 2-associating factor 1 antibody
      • Pou2af1 antibody
      see all

    Anti-BOB1 antibody [SP92] images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOB1 antibody [SP92] (ab99891)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOB1 antibody [SP92] (ab99891)
      Staining of Human BOB1 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab99891 at a dilution of 1/100.
    • Western blot - BOB1 antibody [SP92] (ab99891)
      Western blot - BOB1 antibody [SP92] (ab99891)
      Anti-BOB1 antibody [SP92] (ab99891) at 1/100 dilution + lysate prepared from Ramos cells

      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    References for Anti-BOB1 antibody [SP92] (ab99891)

    ab99891 has not yet been referenced specifically in any publications.

    Publishing research using ab99891? Please let us know so that we can cite the reference in this datasheet.

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