- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-BOB1 antibody [SP92]
See all BOB1 primary antibodies -
DescriptionRabbit monoclonal [SP92] to BOB1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human BOB1 (C terminal). The exact sequence is proprietary.
Database link: Q16633 -
Positive control
- Human Tonsil
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General notes
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 -
Concentration information loading... -
Purification notesPurified from TCS by protein A/G.
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ClonalityMonoclonal
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Clone numberSP92
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab99891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| IHC-P | 1/100. Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
Target
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FunctionTranscriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
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Tissue specificityB-cell specific.
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Involvement in diseaseNote=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
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Sequence similaritiesBelongs to the POU2AF1 family.
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Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5450 Human
- Omim: 601206 Human
- SwissProt: Q16633 Human
- Unigene: 654525 Human
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Alternative names
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (ab99891)](http://a.abcam.com/ps/products/99/ab99891/Images/BOB1-Primary-antibodies-ab99891-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (ab99891)Staining of Human BOB1 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab99891 at a dilution of 1/100. -
![Western blot - Anti-BOB1 antibody [SP92] (ab99891)](http://a.abcam.com/ps/products/99/ab99891/Images/BOB1-Primary-antibodies-ab99891-2.jpg)
Western blot - Anti-BOB1 antibody [SP92] (ab99891)Anti-BOB1 antibody [SP92] (ab99891) at 1/100 dilution + lysate prepared from Ramos cells
Observed band size: 35 kDa (why is the actual band size different from the predicted?)
References
ab99891 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"