Recombinant
Anti-BOB1 antibody [SP92], prediluted (ab100903)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-BOB1 antibody [SP92], prediluted
See all BOB1 primary antibodies -
DescriptionRabbit monoclonal [SP92] to BOB1, prediluted
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide derived from the C terminal region of Human BOB1
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Positive control
- Human tonsil tissue
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General notes
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormPrediluted
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6 -
Concentration information loading... -
Purification notesPurified from TCS by protein A/G.
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ClonalityMonoclonal
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Clone numberSP92
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab100903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1. | |
| IHC-P | 1/1. |
Target
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FunctionTranscriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
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Tissue specificityB-cell specific.
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Involvement in diseaseNote=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
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Sequence similaritiesBelongs to the POU2AF1 family.
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Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5450 Human
- Omim: 601206 Human
- SwissProt: Q16633 Human
- Unigene: 654525 Human
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Alternative names
- B cell Oct binding protein 1 antibody
- B cell specific coactivator OBF 1 antibody
- B cell specific coactivator OBF1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOB1 antibody [SP92], prediluted (ab100903)](http://a.abcam.com/ps/datasheet/images/100/ab100903/BOB1-Primary-antibodies-ab100903-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BOB1 antibody [SP92], prediluted (ab100903)Staining of BOP1 in formalin-fixed, paraffin-embedded Human tonsil tissue using prediluted ab100903. -
![Western blot - BOB1 antibody [SP92], prediluted (ab100903)](http://a.abcam.com/ps/datasheet/images/100/ab100903/BOB1-Primary-antibodies-ab100903-3.jpg)
Western blot - BOB1 antibody [SP92], prediluted (ab100903)Anti-BOB1 antibody [SP92] (ab99891) at 1/100 dilution + lysate prepared from Ramos cells
Observed band size: 35 kDa (why is the actual band size different from the predicted?)
ab99891 used in the image is the undiluted version of ab100903.
References
ab100903 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"