Overview

  • Product name
  • Description
    Goat polyclonal to BOC
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    C-TQEVKTSGSSDRLR

    , corresponding to internal sequence amino acids 207-220 of human BOC (NP_150279.1).

  • Positive control
    • Human heart and ileum lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab77556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 121 kDa.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Brother of CDO (BOC) interacts with Hedgehog (Hh) via a specific FNIII domain and belongs to the Ihog (interference hedgehog) family of proteins. Zebrafish BOC is expressed predominantly in the developing neural plate and later in the neural tube and developing brain.
  • Cellular localization
    Cell membrane; Single pass type I membrane protein. Note: Enriched at sites of cell-cell contact.
  • Database links
  • Alternative names
    • BOC cell adhesion associated oncogene regulated antibody
    • Boc homolog (mouse) antibody
    • Boc homolog antibody
    • Brother of CDO antibody
    • Brother of CDON antibody
    • CDON2 antibody
    • Cell adhesion associated oncogene regulated 2 antibody
    • Protein BOC antibody
    see all

Anti-BOC antibody images

  • Anti-BOC antibody (ab77556) at 0.3 µg/ml + human ileum lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 121 kDa
    Observed band size : 121 kDa


    Exposure time : 1 hour
  • ab77556 staining BOC in Human smotth muscle cells by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 100% antibody diluent for 30 minutes at room temperature; antigen retrieval was with Proteinase K + Citrate Buffer pH 6. Samples were incubated with primary antibody (1/200) for 12 hours at 4°C. An undiluted HRP-conjugated Mouse anti-goat monocloanl was used as the secondary antibody.

    See Abreview

References for Anti-BOC antibody (ab77556)

ab77556 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Smooth muscle cells in Aorta, Intestine Umbilical)
Specification
Smooth muscle cells in Aorta, Intestine Umbilical
Fixative
Formaldehyde
Antigen retrieval step
Other
Permeabilization
No
Blocking step
Antibody diluent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Thomas Quentin

Verified customer

Submitted Aug 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up