Overview

  • Product name
  • Description
    Rabbit polyclonal to Bod1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 134-163 of Human Bod1

  • Positive control
    • MCF-7 cell line lysates Brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab103510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for proper chromosome biorientation through the detection or correction of syntelic attachments in mitotic spindles.
  • Sequence similarities
    Belongs to the BOD1 family.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Localizes at the centrosomes throughout the cell cycle, only dissociating during cytokinesis. Localizes at the kinetochore from prometaphase until anaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Biorientation defective 1 antibody
    • Biorientation defective protein 1 antibody
    • biorientation of chromosomes in cell division 1 antibody
    • Biorientation of chromosomes in cell division protein 1 antibody
    • BOD1 antibody
    • BOD1_HUMAN antibody
    • FAM44B antibody
    • family with sequence similarity 44, member B antibody
    • Protein FAM44B antibody
    see all

Images

  • Anti-Bod1 antibody (ab103510) at 1/100 dilution + MCF-7 cell line lysates at 35 µg

    Predicted band size : 19 kDa
  • IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.ab103510 used at 1:50 dilution.

References

ab103510 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Tissue lysate - whole (Embryonic Brain)
Specification
Embryonic Brain
Negative control
IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 90 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Paraformaldehyde
Positive control
Comparison to H3K27me2 enrichment at Dlx5/6 intergenic region.
Username

Abcam user community

Verified customer

Submitted Dec 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell lysate)
Loading amount
35 µg
Specification
HeLa cell lysate
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted May 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell)
Loading amount
25 µg
Specification
HeLa cell
Gel Running Conditions
Reduced Denaturing (4-12% NuPAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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