Overview

  • Product nameAnti-Bok antibody
    See all Bok primary antibodies
  • Description
    Rabbit polyclonal to Bok
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the N-terminal region (BH3 domain) of human Bok.

  • Positive control
    • HL-60 cell lysate; Parraffin embedded human hepatocarcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71646 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

Target

Anti-Bok antibody images

  • Anti-Bok antibody (ab71646) at 1/100 dilution + HL-60 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • ab71646 at 1/50 dilution, staining Bok in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-Bok antibody (ab71646)

ab71646 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Hepatocytes and Bone marrow cells)
Loading amount 50 µg
Specification Hepatocytes and Bone marrow cells
Gel Running Conditions Reduced Denaturing (4-20% Tris Glycine Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"