Overview

  • Product nameAnti-BOLA1 antibody
    See all BOLA1 primary antibodies
  • Description
    Mouse polyclonal to BOLA1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human BOLA1 protein (NP_057158.1).

  • Positive control
    • WB: Hela cell lysate and BOLA1 transfected 293T cell lysate ICC/IF: Hela cell

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 14 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the bolA/yrbA family.
  • Cellular localizationSecreted. Could be secreted via a non-classical export pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOLA 1 antibody
    • BolA homolog 1 antibody
    • BolA like 1 antibody
    • BolA like protein 1 antibody
    • BolA-like protein 1 antibody
    • Bola1 antibody
    • BOLA1_HUMAN antibody
    • CGI 143 antibody
    • hBolA antibody
    • MGC75015 antibody
    • RP11 196G18.18 antibody
    see all

Anti-BOLA1 antibody images

  • Anti-BOLA1 antibody (ab88307) at 1 µg/ml + Hela cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
  • All lanes : Anti-BOLA1 antibody (ab88307) at 1 µg/ml

    Lane 1 : BOLA1 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 14 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)
  • ab88307, at 10 µg/ml, staining BOLA1 in HeLa cell by Immunofluorescence.

References for Anti-BOLA1 antibody (ab88307)

ab88307 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"