Overview

  • Product nameAnti-Bone Sialoprotein antibody
    See all Bone Sialoprotein primary antibodies
  • Description
    Rabbit polyclonal to Bone Sialoprotein
  • Tested applicationsSuitable for: ELISA, RIA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Human Bone Sialoprotein (bone extract)

Properties

Applications

Our Abpromise guarantee covers the use of ab52128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
  • Post-translational
    modifications
    N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
    O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
    Sulfated on either Tyr-313 or Tyr-314.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BNSP antibody
    • Bone sialoprotein 2 antibody
    • Bone sialoprotein II antibody
    • BSP antibody
    • BSP II antibody
    • BSPII antibody
    • Cell binding sialoprotein antibody
    • Cell-binding sialoprotein antibody
    • IBSP antibody
    • Integrin binding sialoprotein antibody
    • Integrin-binding sialoprotein antibody
    • SIAL_HUMAN antibody
    • SPII antibody
    see all

Anti-Bone Sialoprotein antibody images

  • ab52128 (2ug/ml) staining Bone Sialoprotein in human bone using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Bone sialoprotein in sectretory compartments in the cells of the perichondrium
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References for Anti-Bone Sialoprotein antibody (ab52128)

This product has been referenced in:
  • Bojar W  et al. Bone regeneration potential of the new chitosan-based alloplastic biomaterial. J Biomater Appl 28:1060-8 (2014). Rat . Read more (PubMed: 23796631) »
  • Gamblin AL  et al. Osteoblastic and osteoclastic differentiation of human mesenchymal stem cells and monocytes in a miniaturized three-dimensional culture with mineral granules. Acta Biomater 10:5139-47 (2014). Read more (PubMed: 25196309) »

See all 2 Publications for this product

Product Wall

Thank you very much for your reply.

The replacement vial of ab125227 will ship out with our next delivery to Biogen. Due to Customs restrictions, this replacement will ship out at the full price ($303.30) but I have arranged credit note # ***...

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Thank you very much for your reply.

I am sorry to hear that the results are still not satisfactory, and I appreciate how much time the customer has spenttroubleshooting. It sounds like the antibody is not work as expected, and I would be happ...

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Thank you very much for your reply and for sending the additional information.

We have not tried the microwave method with this antibody, but one of the other methods (steamer or pressure cooker) may work better. I would suggest using the ste...

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Thank you very much for contacting us and letting us know about the customer's trouble with this antibody.

Threemonths in EDTA is not too long for decalcification. Bone can be a difficult tissue to work with, soit's possible that there is a p...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Endochondral bone)
Specification Endochondral bone
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
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Verified customer

Submitted Jan 25 2011

Thank you for your inquiry. Unfortunatly we do not have a specific dilution to recommend for these antibodies. I can suggest to try a range of dilutions from 1:100 to 1:3000 and to optimize the dilution according to the results. Too much antibody w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"