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Rabbit polyclonal to BOP1
Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residue 300 and 350 of Human BOP1 (NP_056016.1).
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
Use at 2-5 µg/mg of lysate.
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Belongs to the WD repeat BOP1/ERB1 family.
Contains 7 WD repeats.
Nucleus > nucleolus. Nucleus > nucleoplasm.
Information by UniProt
Block of proliferation 1 antibody
Western blot - BOP1 antibody (ab86653)
All lanes :
Anti-BOP1 antibody (ab86653) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Predicted band size :
Observed band size :
100,110 kDa (
why is the actual band size different from the predicted?
Additional bands at :
120 kDa,65 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - BOP1 antibody (ab86653)
Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 90 seconds
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"