Anti-BOP1 antibody (ab86653)
Key features and details
- Rabbit polyclonal to BOP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BOP1 antibody
See all BOP1 primary antibodies -
Description
Rabbit polyclonal to BOP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to a region between residue 300 and 350 of Human BOP1 (NP_056016.1).
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 84 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
Sequence similarities
Belongs to the WD repeat BOP1/ERB1 family.
Contains 7 WD repeats. -
Cellular localization
Nucleus > nucleolus. Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23246 Human
- Omim: 610596 Human
- SwissProt: Q14137 Human
- Unigene: 645279 Human
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Alternative names
- AU020183 antibody
- AW146150 antibody
- Block of proliferation 1 antibody
see all
Images
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All lanes : Anti-BOP1 antibody (ab86653) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 84 kDa
Observed band size: 100,110 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 65 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure time of 90 seconds -
Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 90 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab86653 has not yet been referenced specifically in any publications.