Western Blot detection of BOP1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86653 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 90 seconds
References for Anti-BOP1 antibody (ab86653)
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