Anti-Bordetella pertussis antibody [BDI500] (ab24212)

Overview

  • Product nameAnti-Bordetella pertussis antibody [BDI500]
  • Description
    Mouse monoclonal [BDI500] to Bordetella pertussis
  • SpecificitySpecific for LOS-A of B. pertussis and B. bronchiseptica.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with Bordetella pertussis. Not yet tested in other species.
  • Immunogen

    Bordetella pertussis N-lauroyl sarcosine membrane extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure.
  • ClonalityMonoclonal
  • Clone numberBDI500
  • IsotypeIgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
WB
  • Application notesELISA: 1/20 - 1/200. IF: 1/10 - 1/50. WB: 1/10 - 1/50. Dilution optimised using Chromogenic detection.
  • Target

    • RelevanceBordetella pertussis is an aerobic, gram negative coccobacilli. It causes whooping cough (pertussis), an acute respiratory infection marked by severe, spasmodic coughing episodes during the paroxysmal phase. Leukocytosis with lymphocytosis is also common during this phase of the illness. Dangerous complications are bronchopneumonia and acute encephalopathy. It produces a number of virulence factors, including pertussis toxin, adenylate cyclase toxin, filamentous hemagglutinin, and hemolysin. Agglutinogens and other outer membrane proteins are important antigens. Pertussis toxin (islet activating protein) is the major protein toxin produced by virulent strains of Bordetella pertussis. As revealed by polyacrylamide gel electrophoresis, the purified protein consists of five dissimilar subunits: S1 (MW 28,000), S2 (MW 23,000), S3 (MW 22,000), S4 (MW 11,700) and S5 (MW 9,300), in a molar ratio of 1:1:1:2:1. The A protomer, S1 is responsible for the enzymatic activity of the toxin. Together, S2, S3, S4 and S5 comprise the B oligomer, responsible for binding the toxin to the cell surface.
    • Alternative names
      • B.pertussis antibody

    References for Anti-Bordetella pertussis antibody [BDI500] (ab24212)

    ab24212 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. We have one antibody raised against B. pertussis but it has not been tested in IHC on paraffin-embedded tissue. It may work in this application, but our guarantee does not cover IHC. Here is a link to the dat...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"