Anti-Bordetella pertussis toxin antibody (ab188414)

Overview

  • Product name
    Anti-Bordetella pertussis toxin antibody
    See all Bordetella pertussis toxin primary antibodies
  • Description
    Rabbit polyclonal to Bordetella pertussis toxin
  • Tested applications
    Suitable for: Dot blot, IP, Neutralising, ELISA, WBmore details
  • Species reactivity
    Reacts with: Bordetella pertussis
  • Immunogen

    Full length native protein (purified) corresponding to Bordetella pertussis toxin. (Immunization was initiated with toxoid and boosted with native toxin).

  • Positive control
    • Culture medium of Bordetella pertussis; Purified Bordetella pertussis toxin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab188414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/2000 - 1/10000.
IP 1/200 - 1/500.
Neutralising Use at an assay dependent concentration.
ELISA 1/10000 - 1/20000.
WB 1/2000 - 1/10000.

Target

  • Relevance
    Pertussis toxin contains five different chains, S1-S5. They are organized into 2 functional subunits: A, composed of S1 (which is toxic) and B, containing S2, S3, S5, and two copies of S4 (B binds to the membrane receptors). Dimers of S2-S4 and S3-S4 are held together by S5
  • Database links
    • Alternative names
      • IAP S1 antibody
      • Islet-activating protein S1 antibody
      • NAD-dependent ADP-ribosyltransferase antibody

    Images

    • All lanes : Anti-Bordetella pertussis toxin antibody (ab188414) at 1/2000 dilution

      Lane 1 : Culture medium of Bordetella pertussis
      Lane 2 : Purified Bordetella pertussis toxin at 0.01 µg

      The toxin consists of five subunits as indicated by S1 to S5.

    • ELISA analysis of Bordetella pertussis toxin, using ab188414. Plate was coated with 100 μg of pertussis toxin per well and 100 μl of ab188414 at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Color was developed with TMB as substrate.

    • ELISA analysis of Bordetella pertussis toxin, using ab188414 at 1/12500 dilution. Plate was coated with indicated amounts of pertussis toxin per well and 100 μl of ab188414 was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as a secondary antibody. Color was developed with TMB as substrate. Dynamic range was 100 pg to 10 ng under these conditions.

    References

    ab188414 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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