Anti-Borealin/CDCA8 antibody (ab67126)

Overview

  • Product nameAnti-Borealin/CDCA8 antibody
    See all Borealin/CDCA8 primary antibodies
  • Description
    Mouse polyclonal to Borealin/CDCA8
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native Borealin/CDCA8 (purified) (Human)

  • Positive control
    • Borealin/CDCA8 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use at an assay dependent dilution. PFA fixation is necessary

Target

  • FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • Sequence similaritiesBelongs to the borealin family.
  • Developmental stageCell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • DomainThe C-terminal region (aa 207-280) represents the dimerization motif.
  • Post-translational
    modifications
    Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • Cellular localizationNucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOR antibody
    • BOREA_HUMAN antibody
    • Borealin antibody
    • CDCA8 antibody
    • Cell division cycle associated 8 antibody
    • cell division cycle associated protein 8 antibody
    • Cell division cycle-associated protein 8 antibody
    • Dasra B antibody
    • Dasra-B antibody
    • DasraB antibody
    • FLJ10468 antibody
    • FLJ12042 antibody
    • hDasra B antibody
    • hDasra-B antibody
    • MESRGP antibody
    • OTTHUMP00000004514 antibody
    • pluripotent embryonic stem cell related gene 3 protein antibody
    • Pluripotent embryonic stem cell-related gene 3 protein antibody
    see all

Anti-Borealin/CDCA8 antibody images

  • All lanes : Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilution

    Lane 1 : Borealin/CDCA8 transfected 293T cell lysate
    Lane 2 : Untransfected 293T cell lysate

    Secondary
    Goat anti mouse IgG (H&L)-HRP conjugate secondary antibody at 1/2500 dilution

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
  • ab67126 staining Borealin/CDCA8 in HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 10% BSA for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 23°C. The secondary antibody used was a mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/300 dilution.
    When fixed in PFA, the antibody stains the centromere in metaphase and the midzone in anaphase, as expected for Borealin, a CPC protein. Borealin (red), Kinetochores (green), DAPI (blue).

    See Abreview

References for Anti-Borealin/CDCA8 antibody (ab67126)

ab67126 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 15 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Apr 19 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Chicken Cell lysate - whole cell (DF1)
Loading amount 15 µg
Specification DF1
Gel Running Conditions Reduced Non-Denaturing (Native)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Mar 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa (Human cervical cancer cells))
Specification HeLa (Human cervical cancer cells)
Fixative Paraformaldehyde
Permeabilization Yes - PBS 0.1% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Feb 25 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Verified customer

Submitted Feb 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"