The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Use at an assay dependent dilution. PFA fixation is necessary
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Belongs to the borealin family.
Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
The C-terminal region (aa 207-280) represents the dimerization motif.
Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
pluripotent embryonic stem cell related gene 3 protein antibody
Pluripotent embryonic stem cell-related gene 3 protein antibody
Western blot - Anti-Borealin/CDCA8 antibody (ab67126)
All lanes : Anti-Borealin/CDCA8 antibody (ab67126) at 1/500 dilution
Lane 1 : Borealin/CDCA8 transfected 293T cell lysate Lane 2 : Untransfected 293T cell lysate
Secondary All lanes : Goat anti mouse IgG (H&L)-HRP conjugate secondary antibody at 1/2500 dilution
Predicted band size: 31 kDa Observed band size: 31 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Borealin/CDCA8 antibody (ab67126)Image courtesy of an anonymous Abreview.
ab67126 staining Borealin/CDCA8 in HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 10% BSA for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 23°C. The secondary antibody used was a mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/300 dilution. When fixed in PFA, the antibody stains the centromere in metaphase and the midzone in anaphase, as expected for Borealin, a CPC protein.
Borealin (red), Kinetochores (green), DAPI (blue).