Overview

  • Product name
    Anti-Borealin/CDCA8 antibody
    See all Borealin/CDCA8 primary antibodies
  • Description
    Rabbit polyclonal to Borealin/CDCA8
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the center region of human Borealin/CDCA8

  • Positive control
    • HL60 cytoplasmic or nuclear lysates; Paraffin embedded Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab70910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Function
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • Sequence similarities
    Belongs to the borealin family.
  • Developmental stage
    Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • Domain
    The C-terminal region (aa 207-280) represents the dimerization motif.
  • Post-translational
    modifications
    Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOR antibody
    • BOREA_HUMAN antibody
    • Borealin antibody
    • CDCA8 antibody
    • Cell division cycle associated 8 antibody
    • cell division cycle associated protein 8 antibody
    • Cell division cycle-associated protein 8 antibody
    • Dasra B antibody
    • Dasra-B antibody
    • DasraB antibody
    • FLJ10468 antibody
    • FLJ12042 antibody
    • hDasra B antibody
    • hDasra-B antibody
    • MESRGP antibody
    • OTTHUMP00000004514 antibody
    • pluripotent embryonic stem cell related gene 3 protein antibody
    • Pluripotent embryonic stem cell-related gene 3 protein antibody
    see all

Anti-Borealin/CDCA8 antibody images

  • Anti-Borealin/CDCA8 antibody (ab70910) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    Additional bands at : 18 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab70910, at a 1/10 dilution, staining human Borealin/CDCA8 in lung carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70910 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References for Anti-Borealin/CDCA8 antibody (ab70910)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton in PBS
Specification
HeLa
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Submitted Apr 06 2016

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