The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
1/10 - 1/50.
FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
Sequence similaritiesBelongs to the borealin family.
Developmental stageCell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
DomainThe C-terminal region (aa 207-280) represents the dimerization motif.
Post-translational modificationsPhosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
Cellular localizationNucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
ab70910, at a 1/10 dilution, staining human Borealin/CDCA8 in lung carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue. ab70910 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
References for Anti-Borealin/CDCA8 antibody (ab70910)