Anti-Borealin/CDCA8 antibody (ab74473)


  • Product name
    Anti-Borealin/CDCA8 antibody
    See all Borealin/CDCA8 primary antibodies
  • Description
    Rabbit polyclonal to Borealin/CDCA8
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide of 19 amino acids taken from near the N-terminus of Human Borealin/CDCA8. (NP_060571)

  • Positive control
    • Rat kidney tissue lysate



Our Abpromise guarantee covers the use of ab74473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 31 kDa).


  • Function
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.
  • Sequence similarities
    Belongs to the borealin family.
  • Developmental stage
    Cell-cycle regulated. Increases during G2/M phase and then reduces after exit from M phase.
  • Domain
    The C-terminal region (aa 207-280) represents the dimerization motif.
  • Post-translational
    Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
    Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
  • Cellular localization
    Nucleus > nucleolus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Chromosome > centromere. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOR antibody
    • BOREA_HUMAN antibody
    • Borealin antibody
    • CDCA8 antibody
    • Cell division cycle associated 8 antibody
    • cell division cycle associated protein 8 antibody
    • Cell division cycle-associated protein 8 antibody
    • Dasra B antibody
    • Dasra-B antibody
    • DasraB antibody
    • FLJ10468 antibody
    • FLJ12042 antibody
    • hDasra B antibody
    • hDasra-B antibody
    • MESRGP antibody
    • OTTHUMP00000004514 antibody
    • pluripotent embryonic stem cell related gene 3 protein antibody
    • Pluripotent embryonic stem cell-related gene 3 protein antibody
    see all

Anti-Borealin/CDCA8 antibody images

  • Lane 1 : Anti-Borealin/CDCA8 antibody (ab74473) at 1 µg/ml
    Lane 2 : Anti-Borealin/CDCA8 antibody (ab74473) at 2 µg/ml

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat kidney lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 31 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of CDCA8 in Rat Kidney cells using ab74473 at 20 ug/ml.

References for Anti-Borealin/CDCA8 antibody (ab74473)

ab74473 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Purified protein (GST-Borealin of human origin)
Loading amount
1 µg
GST-Borealin of human origin
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

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Submitted Apr 07 2009


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