Overview

  • Product name
  • Description
    Rabbit polyclonal to BORIS
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide:

    LSEQFTKIKELELMPEKG

    , corresponding to N terminal amino acids 9-26 of Human BORIS.

  • Positive control
    • Human spleen and Human prostate tissue lysates. FFPE mouse testis tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab126766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA 1/2000 - 1/10000.
WB 1/200 - 1/2000. Predicted molecular weight: 75 kDa.

Target

  • Function
    Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
  • Tissue specificity
    Testis specific. Specifically expressed in primary spermatocytes.
  • Sequence similarities
    Belongs to the CTCF zinc-finger protein family.
    Contains 11 C2H2-type zinc fingers.
  • Developmental stage
    Is up-regulated in primary spermatocytes (expression overlapping with the genome-wide erasure of methylation) to become silenced on activation of CTCF in post-meiotic germline cells (expression corresponding to DNA remethylation).
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BORIS like protein antibody
    • Brother of the regulator of imprinted sites antibody
    • Cancer/testis antigen 27 antibody
    • CCCTC binding factor (zinc finger protein) like antibody
    • CCCTC-binding factor antibody
    • CT27 antibody
    • CTCF paralog antibody
    • CTCF T antibody
    • CTCF-like protein antibody
    • Ctcfl antibody
    • CTCFL_HUMAN antibody
    • dJ579F20.2 antibody
    • HMG 1L1 antibody
    • HMGB1L1 antibody
    • MGC163358 antibody
    • MGC169105 antibody
    • MGC169106 antibody
    • Putative high mobility group protein 1 like 1 antibody
    • Putative high mobility group protein B1 like 1 antibody
    • Transcriptional repressor CTCFL antibody
    • Zinc finger protein CTCF-T antibody
    see all

Images

  • All lanes : Anti-BORIS antibody (ab126766) at 1/200 dilution

    Lane 1 : Human prostate tissue lysate
    Lane 2 : Human spleen tissue lysate


    Predicted band size : 75 kDa
  • IHC image of BORIS staining in mouse testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126766, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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