The product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/200. Reactive Western Blot bands at 83kD, 41kD, 34kD and 31kD + additional low MW bands
1/20 - 1/200.
1/200 - 1/1000.
Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response.
B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.