Anti-Borrelia burgdorferi antibody (HRP) (ab20757)

Overview

  • Product name
    Anti-Borrelia burgdorferi antibody (HRP)
    See all Borrelia burgdorferi primary antibodies
  • Description
    Rabbit polyclonal to Borrelia burgdorferi (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with Treponema pallidum, B. hermsii and B. parkerii. Not yet tested in other species.
  • Immunogen

    Whole cell preparation from B. burgdorferi.

  • General notes

    Purified IgG fraction of antiserum covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA
    Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-P
  • Application notes
    ELISA: 1/200 - 1/1000.
    IHC-P: 1/20 - 1/200.
    WB: 1/20 - 1/200. Detects bands of approximately 83 kDa, 41 kDa, 34 kDa, 31 kDa and additional low MW bands. Dilution optimised using Chromogenic detection.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.
    • Alternative names
      • B burgdorferi antibody
      • B. burgdorferi antibody
      • B.burgdorferi antibody
      • Lyme disease antibody

    References

    ab20757 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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