Reacts with Treponema pallidum, B. hermsii and B. parkerii.
Not yet tested in other species.
Whole cell preparation from B. burgdorferi.
Purified IgG fraction of antiserum covalently coupled to a highly purified preparation of Horseradish Peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.002% Thimerosal (merthiolate)
Constituents: PBS, 10mg/ml BSA
Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/200 - 1/1000.
IHC-P: 1/20 - 1/200.
WB: 1/20 - 1/200. Detects bands of approximately 83 kDa, 41 kDa, 34 kDa, 31 kDa and additional low MW bands. Dilution optimised using Chromogenic detection.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceBorrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response.
B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.
B burgdorferi antibody
B. burgdorferi antibody
Lyme disease antibody
References for Anti-Borrelia burgdorferi antibody (HRP) (ab20757)
has not yet been referenced specifically in any publications.
Publishing research using ab20757? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"