Overview

  • Product nameAnti-BOULE antibody
    See all BOULE primary antibodies
  • Description
    Goat polyclonal to BOULE
  • Tested applicationsSuitable for: ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    (C)QPEPIKTVWSIHY

    , corresponding to C terminal amino acids 271-283 of Human BOULE

  • Positive control
    • IHC-P: FFPE human testis.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 4 - 6 µg/ml.

Target

  • FunctionProbable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
  • Tissue specificityTestis specific. Not expressed in early embryos, primordial germ cells and spermatogonial cells. First expressed in the cytoplasm of spermatocytes and then persists through meiosis.
  • Sequence similaritiesBelongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bol (Drosophila boule homolog) like antibody
    • Bol antibody
    • Bol boule like antibody
    • Bol, boule like (Drosophila) antibody
    • bol, boule-like (Drosophila) antibody
    • Boll antibody
    • BOLL_HUMAN antibody
    • BOULE antibody
    • Boule like antibody
    • BOULE, Drosophila, homolog of antibody
    • Protein boule like antibody
    • Protein boule-like antibody
    • Putative uncharacterized protein BOLL antibody
    see all

Anti-BOULE antibody images

  • IHC image of BOULE staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28745, 1µg/ml, for 15 mins at room temperature. A donkey anti-goat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. ab28745 (4µg/ml) staining following steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

References for Anti-BOULE antibody (ab28745)

ab28745 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"