Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab1072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceThe bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
    • Cellular localizationHost cell nucleus
    • Alternative names
      • BPVgp4 antibody
      • E2 antibody
      • Regulatory protein E2 antibody

    References for Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)

    ab1072 has not yet been referenced specifically in any publications.

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    I'm sorry to hear you are having problems with ab1072. I am currently trying to find out more information about the protocol used to test the antibody in WB. Have you tested the blot without milk blocking and if the non specific bands could be due ...

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