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Our Abpromise guarantee covers the use of ab9092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.
Detection occurs using a 1:5000 dilution of IRDye800TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.