Anti-Bovine Serum Albumin antibody [5F9] (ab9092)

Overview

  • Product nameAnti-Bovine Serum Albumin antibody [5F9]
    See all Bovine Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [5F9] to Bovine Serum Albumin
  • SpecificityThis antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Cow
    Does not react with: Chicken, Human
  • General notesThis product contains 0.02% (w/v) Sodium Azide preservative.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number5F9
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesThis product was assayed by immunoblot using IRDye800TM Goat-anti-Mouse IgG.
    A working dilution of 1:5,000 is suggested for this assay.
    Researchers should determine optimal titers for other applications.
    The antibody may be suitable for other applications, although no supporting data is available
    ELISA: Use at an assay dependent dilution.
  • Target

    • RelevanceAlbumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • ALB antibody
      • Albumin (32 AA) antibody
      • Albumin (AA 34) antibody
      • Albumin antibody
      • BSA antibody
      • Cell growth inhibiting protein 42 antibody
      • Growth inhibiting protein 20 antibody
      • PRO0883 antibody
      • PRO0903 antibody
      • PRO1341 antibody
      • Serum albumin antibody
      see all

    Anti-Bovine Serum Albumin antibody [5F9] images

    • Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.

      Detection occurs using a 1:5000 dilution of IRDye800TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.

    References for Anti-Bovine Serum Albumin antibody [5F9] (ab9092)

    This product has been referenced in:
    • Li X  et al. Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran. Colloids Surf B Biointerfaces 61:113-7 (2008). Read more (PubMed: 17703925) »

    See 1 Publication for this product

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    Please type Bovine Serum Album in the search box or click on the following link

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    I hope this information is helpful to you. Please do n...

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    Ab9092 was purified from ascites.

    Thank you for your enquiry. We would like to confirm that this antibody derived from ascites.

    Albumin is the most abundant protein in serum. I don't know what dilutions you did to human serum? The antibody would non-specifically bind to HSA when HSA overwhelmingly presented in the loading sample. I suggest you try to dilute both sera at least 1...

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    Thank you for your message. We would suggest trying PBS with 0.1% Tween 20 and 1.5% fish gel as blocking and dilution buffer. Load 20uL of purified BSA at 0.5mg/mL and use 1:1000 dilution of the primary antibody.

    For the protocol please reference our ELISA under Methods on the web. The BSA we've been using is Fraction V (IgG and Protease free).

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"