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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Bovine Serum Albumin antibody (Biotin)
See all Bovine Serum Albumin products (12) ...
Description
Rabbit polyclonal to Bovine Serum Albumin (Biotin)
Conjugation
Biotin
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum].
Tested applications
Immunomicroscopy, WB, Dot Blot, ELISA, IHC-Frmore details
Cross reactivity
Reacts with
Cow
Immunogen
Full length native Albumin (purified).
Properties
Form
Liquid
Storage instructions
Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml Polyethylene Glycol, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunomicroscopy
WB
Dot Blot
ELISA
IHC-Fr
Application notes
Dot: Use at an assay dependant dilution.
ELISA: 1/4000 - 1/20000.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Cellular localization
Secreted
Alternative names
- ALB antibody
- Albumin antibody
- Allergen Bos d 6 antibody
see all
Database links
References for Anti-Bovine Serum Albumin antibody (Biotin) (ab7636)
This product has been referenced in:
- Tsuzuki Set al. Photo-immobilization of biological components on gold-coated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM). Biotechnol Bioeng 102:700-7 (2009).Read more (PubMed: 18989902) »
- Prosperi Det al. Avidin decorated core-shell nanoparticles for biorecognition studies by elastic light scattering. Chembiochem 8:1021-8 (2007).Read more (PubMed: 17503421) »
See all 2 publications for this product
Publishing research using ab7636? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"