Bovine Serum Albumin antibody (Biotin) (ab7636)

Product Name 

Bovine Serum Albumin antibody (Biotin)

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Bovine Serum Albumin (Biotin)

Immunogen 

Full length native Albumin (purified).

Reacts with 
(species key)

Cow

Specificity 

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum].

Tested applications 
(see key)

Dot, ELISA, IHC-Fr, IM, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependant dilution.
ELISA: 1/4000 - 1/20000.
IHC-Fr: Use at an assay dependant dilution.
IM: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cardiovascular >> Blood >> Serum Proteins

Relevance 

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume.
Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Conjugation notes 

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml Polyethylene Glycol, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.

Form 

Liquid

Conjugation 

Biotin

Concentration 

10.000 mg/ml

Storage instructions 

Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

References for Bovine Serum Albumin antibody (Biotin) (ab7636)

  This product has been used in: (two most recent references)

Tsuzuki S et al. Photo-immobilization of biological components on gold-coated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM). Biotechnol Bioeng 102:700-7 (2009). PubMed: 18989902

Prosperi D et al. Avidin decorated core-shell nanoparticles for biorecognition studies by elastic light scattering. Chembiochem 8:1021-8 (2007). PubMed: 17503421
If you publish research using ab7636 please let us know so that we can cite the reference on this datasheet.

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