Anti-Bovine Serum Albumin antibody [BSA-7G10] (ab3781)

Overview

  • Product nameAnti-Bovine Serum Albumin antibody [BSA-7G10]
    See all Bovine Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [BSA-7G10] to Bovine Serum Albumin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow
    Predicted to work with: Rabbit, Human
  • Immunogen

    Full length native protein (purified) (Cow).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberBSA-7G10
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/10000.
WB 1/1200 - 1/2000.

Target

  • RelevanceAlbumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • ALB antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • BSA antibody
    • Cell growth inhibiting protein 42 antibody
    • Growth inhibiting protein 20 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

References for Anti-Bovine Serum Albumin antibody [BSA-7G10] (ab3781)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (MEF)
Specification MEF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Jul 15 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (12% resolving, 4.8% stacking gel)
Sample Rabbit Tissue lysate - whole (Heparinized rabbit blood)
Specification Heparinized rabbit blood
Treatment Rabbit blood digested by mosquitoes for 0 & 18hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2014

Thank you for your reply.

I heard back from the lab that ab3781 can be used with this standard protocol:
- Blocking for 20 min to 1 hour with TBST 1X with 5% dry milk
- Wash briefly with TBST 1X
- Incubation with the antibody a...

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Thank you for your inquiry.

I heard back from the lab that this antibody has been successfully tested on BSA in WB. The blocking buffer was TBS-Tween 20 with 5% dry milk. Is this what you had tried?

What dilutions of the antibody ha...

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Thank you for contacting us.

Please type Bovine Serum Album in the search box or click on the following link

http://www.abcam.com/index.html?pageconfig=searchresults

I hope this information is helpful to you. Please do n...

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Thank you for your phone call.
I am very sorry to hear that these antibodies did not work together in sandwich ELISA. I am sending you the following discount codes as compensation for trying the antibodies in an untested application. Unfortunately...

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Thank you for contacting us. These two anti-BSA antibodies are provided as ascites. Monoclonal antibodies can be produced by growing hybridoma cells within the peritoneal cavity of a mouse. When injected into a mouse, the hybridoma cells multiply an...

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Thank you for your enquiry. Unfortunately, epitope mapping was not done on this product, therefore we do not have any information on the exact epitope binding region. I am sorry I can't be of more assistance in this occasion but if there is anyt...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Purified protein (brain)
Loading amount 10 µg
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

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Verified customer

Submitted Oct 16 2006

This antibody is only available in liquid form.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"