Anti-Bovine Serum Albumin antibody [BSA-7G10] (ab3781)

Overview

  • Product name
    Anti-Bovine Serum Albumin antibody [BSA-7G10]
    See all Bovine Serum Albumin primary antibodies
  • Description
    Mouse monoclonal [BSA-7G10] to Bovine Serum Albumin
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow
    Predicted to work with: Rabbit, Human
  • Immunogen

    Full length native protein (purified) (Cow).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Constituents: 1.2114% Tris, 0.7507% Glycine, 2% Sucrose
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    BSA-7G10
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/10000.
WB 1/1200 - 1/2000.

Target

References

This product has been referenced in:
  • Walkup WG  et al. Phosphorylation of synaptic GTPase-activating protein (synGAP) by Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cyclin-dependent kinase 5 (CDK5) alters the ratio of its GAP activity toward Ras and Rap GTPases. J Biol Chem 290:4908-27 (2015). WB . Read more (PubMed: 25533468) »
  • Wagner CS  et al. Intracellular regulation of cross-presentation during dendritic cell maturation. PLoS One 8:e76801 (2013). Read more (PubMed: 24098562) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your reply.

I heard back from the lab that ab3781 can be used with this standard protocol:
- Blocking for 20 min to 1 hour with TBST 1X with 5% dry milk
- Wash briefly with TBST 1X
- Incubation with the antibody a...

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Thank you for your inquiry.

I heard back from the lab that this antibody has been successfully tested on BSA in WB. The blocking buffer was TBS-Tween 20 with 5% dry milk. Is this what you had tried?

What dilutions of the antibody ha...

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Thank you for contacting us.

Please type Bovine Serum Album in the search box or click on the following link

http://www.abcam.com/index.html?pageconfig=searchresults

I hope this information is helpful to you. Please do n...

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Thank you for your phone call.
I am very sorry to hear that these antibodies did not work together in sandwich ELISA. I am sending you the following discount codes as compensation for trying the antibodies in an untested application. Unfortunately...

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Thank you for contacting us. These two anti-BSA antibodies are provided as ascites. Monoclonal antibodies can be produced by growing hybridoma cells within the peritoneal cavity of a mouse. When injected into a mouse, the hybridoma cells multiply an...

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Thank you for your enquiry. Unfortunately, epitope mapping was not done on this product, therefore we do not have any information on the exact epitope binding region. I am sorry I can't be of more assistance in this occasion but if there is anyt...

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This antibody is only available in liquid form.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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