Bovine Serum Albumin (nitrated ) (ab131379)
Key features and details
- Expression system: Native
- Purity: > 96% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Description
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Product name
Bovine Serum Albumin (nitrated )
See all Bovine Serum Albumin proteins and peptides -
Purity
> 96 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Cow -
Sequence
MKWVTFISLLLLFSSAYSRGVFRRDTHKSEIAHRFKDLGEEHFKGLVLIA FSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCK VASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEF KADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGAC LLPKIETMREKVLASSARQRLRCASIQKFGERALKAWSVARLSQKFPKAE FVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKE CCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFL GSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKL KHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVS RSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCC TESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQT ALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVV STQTALA -
Predicted molecular weight
69 kDa -
Amino acids
1 to 607
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab131379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
ab131379 was treated with peroxynitrite which modifies tyrosine by acting as an oxidant to produce 3-nitrotyrosine along the length of the Bovine Serum Albumin protein.
Product was previously marketed under the MitoSciences sub-brand.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.5
Constituents: 0.61% Tris HCl, 15% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
General Info
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Alternative names
- alb
- ALBU_HUMAN
- Albumin
see all - Information by UniProt
Images
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All lanes: Anti-Nitrotyrosine antibody (ab110282) 0.4 µg/mL.
Lane 1: Molecular weight marker
Lane 2: BSA 100 ng
Lane 3: BSA 25 ng
Lane 4: Nitrated BSA 100 ng
Lane 5: Nitrated BSA 50 ng
Lane 6: Nitrated BSA 25 ng
Lane 7: Nitrated BSA 12.5 ng
Lane 8: Nitrated BSA 6.25 ng
Lane 9: Nitrated BSA 3.125 ngSecondary antibody:
Goat polyclonal to Mouse IgG – Pre-Adsorbed (HRP) at 1/6000
developed using the ECL technique.Performed under reducing conditions.
Predicted band size: 69,293 Da
Observed band size: 69 kDaExposure time: 5 minutes
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Lane 1: Nitrated Bovine Serum Albumin protein (50 ng)
Lane 2: Molecular weight marker
Lane 3: Bovine Serum Albumin protein (50 ng)Gel: 4-20% acrylamide separation gel
Predicted band size: 69 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131379 has not yet been referenced specifically in any publications.