Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab1077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 24076025
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

Target

  • Function
    May act as a mediator of the PTEN growth-suppressive signaling pathway. May play a role in developmental processes.
  • Tissue specificity
    Ubiquitously expressed in all fetal tissues examined including heart, brain, liver, and kidney. Also expressed at lower levels in both adult heart and hypertrophic heart.
  • Sequence similarities
    Contains 2 ANK repeats.
    Contains 2 BTB (POZ) domains.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABTB 1 antibody
    • Abtb1 antibody
    • ABTB1_HUMAN antibody
    • Ankyrin repeat and BTB (POZ) domain containing 1 antibody
    • Ankyrin repeat and BTB/POZ domain containing 1 antibody
    • Ankyrin repeat and BTB/POZ domain containing protein 1 antibody
    • Ankyrin repeat and BTB/POZ domain-containing protein 1 antibody
    • EF1ABP antibody
    • Elongation factor 1A binding protein antibody
    • Elongation factor 1A-binding protein antibody
    • MGC20585 antibody
    • PP 2259 antibody
    • PP2259 antibody
    see all

Images



  • Predicted band size : 39 kDa

    ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa  BPOZ in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa BPOZ in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunocytochemical/Immunofluorescence analysis of mouse primary neurons stimulated with 5 μM of MPP+ for 0, 6, 12, and 24 hours labelling BPOZ (green), TH (red) and Nuclei (DAPI - blue). 

    Coverslips containing neurons cultured to 70–80% confluence were fixed with chilled methanol overnight, followed by two rinses with PBS. Samples were blocked with 2% BSA in PBS containing Tween 20 and Triton X-100 for 30 minutes and incubated at room temperature under shaking conditions for 6 hours in PBS containing the ab1077 (1:200), TH (1:1000). After four 15 minute washes in PBS, slides were incubated with Cy2- or Cy5-labelled secondary antibodies (1:200) for 1 hour under shaking conditions. Following four 15 minute washes with PBS, cells were incubated for 4–5 minutes with DAPI (1:10000).

References

This product has been referenced in:
  • Roy A & Pahan K Ankyrin repeat and BTB/POZ domain containing protein-2 inhibits the aggregation of alpha-synuclein: Implications for Parkinson's disease. FEBS Lett 587:3567-74 (2013). ICC/IF ; Mouse . Read more (PubMed: 24076025) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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