Anti-BPTF / FALZ antibody [2343C3a] (ab54356)

Overview

  • Product name
    Anti-BPTF / FALZ antibody [2343C3a]
    See all BPTF / FALZ primary antibodies
  • Description
    Mouse monoclonal [2343C3a] to BPTF / FALZ
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (human) from an internal sequence.

Properties

Applications

Our Abpromise guarantee covers the use of ab54356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 311 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).
  • Sequence similarities
    Belongs to the PBTF family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 2 PHD-type zinc fingers.
  • Developmental stage
    Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).
  • Domain
    The second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine.
  • Post-translational
    modifications
    Phosphorylation enhances DNA-binding.
    Highly susceptible to proteolysis.
  • Cellular localization
    Cytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
  • Information by UniProt
  • Database links
  • Alternative names
    • BPTF antibody
    • BPTF_HUMAN antibody
    • Bromodomain and PHD finger containing transcription factor antibody
    • Bromodomain and PHD finger-containing transcription factor antibody
    • Bromodomain PHD finger transcription factor antibody
    • FAC 1 antibody
    • fac1 antibody
    • FALZ antibody
    • Fetal Alz 50 clone 1 protein antibody
    • Fetal Alz 50 reactive clone 1 antibody
    • Fetal Alz-50 clone 1 protein antibody
    • Fetal Alzheimer antigen antibody
    • Nucleosome remodeling factor antibody
    • Nucleosome remodeling factor subunit BPTF antibody
    • Nucleosome remodeling factor, large subunit antibody
    • Nucleosome-remodeling factor subunit BPTF antibody
    • NURF 301 antibody
    • NURF301 antibody
    see all

Images

  • Anti-BPTF / FALZ antibody [2343C3a] (ab54356) + immunising recombinant protein fragment.

    Predicted band size : 311 kDa

References

ab54356 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (U2-OS cells)
Loading amount
50 µg
Specification
U2-OS cells
Gel Running Conditions
Non-reduced Denaturing (6)
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C
Username

Ms. Ayelet Star

Verified customer

Submitted Feb 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2-OS cells)
Specification
U2-OS cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0.5%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2013

Thank you very much for getting back to me and for confirming the sample type. It is much appreciated.
DISCOUNT CODE: 40403
Expiration date: 04/04/2013
Value: £227.00
I am very pleased to hear you would like to accept our offer and ...

Read More

Thank you for your enquiry and your interest.
We can certainly consider offering a testing discount code. Would you be so kind to discuss with the customer what type human samples (cells, tissue sections, fixation) will be used for testing.
T...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up