Anti-BPTF / FALZ antibody [2343C3a] (ab54356)


  • Product nameAnti-BPTF / FALZ antibody [2343C3a]
    See all BPTF / FALZ primary antibodies
  • Description
    Mouse monoclonal [2343C3a] to BPTF / FALZ
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (human) from an internal sequence.



Our Abpromise guarantee covers the use of ab54356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 311 kDa.
Dot blot Use at an assay dependent dilution.


  • FunctionHistone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
  • Tissue specificityUbiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).
  • Sequence similaritiesBelongs to the PBTF family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 2 PHD-type zinc fingers.
  • Developmental stageAbundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).
  • DomainThe second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine.
  • Post-translational
    Phosphorylation enhances DNA-binding.
    Highly susceptible to proteolysis.
  • Cellular localizationCytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
  • Information by UniProt
  • Database links
  • Alternative names
    • BPTF antibody
    • BPTF_HUMAN antibody
    • Bromodomain and PHD finger containing transcription factor antibody
    • Bromodomain and PHD finger-containing transcription factor antibody
    • Bromodomain PHD finger transcription factor antibody
    • FAC 1 antibody
    • fac1 antibody
    • FALZ antibody
    • Fetal Alz 50 clone 1 protein antibody
    • Fetal Alz 50 reactive clone 1 antibody
    • Fetal Alz-50 clone 1 protein antibody
    • Fetal Alzheimer antigen antibody
    • Nucleosome remodeling factor antibody
    • Nucleosome remodeling factor subunit BPTF antibody
    • Nucleosome remodeling factor, large subunit antibody
    • Nucleosome-remodeling factor subunit BPTF antibody
    • NURF 301 antibody
    • NURF301 antibody
    see all

Anti-BPTF / FALZ antibody [2343C3a] images

  • Anti-BPTF / FALZ antibody [2343C3a] (ab54356) + immunising recombinant protein fragment.

    Predicted band size : 311 kDa

References for Anti-BPTF / FALZ antibody [2343C3a] (ab54356)

ab54356 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (U2-OS cells)
Loading amount 50 µg
Specification U2-OS cells
Gel Running Conditions Non-reduced Denaturing (6)
Blocking step Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C

Ms. Ayelet Star

Verified customer

Submitted Feb 22 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2-OS cells)
Specification U2-OS cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.5%
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 22 2013

Thank you very much for getting back to me and for confirming the sample type. It is much appreciated.
Expiration date: 04/04/2013
Value: £227.00
I am very pleased to hear you would like to accept our offer and ...

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Thank you for your enquiry and your interest.
We can certainly consider offering a testing discount code. Would you be so kind to discuss with the customer what type human samples (cells, tissue sections, fixation) will be used for testing.

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