Overview

  • Product nameAnti-BPTF / FALZ antibody
    See all BPTF / FALZ primary antibodies
  • Description
    Rabbit polyclonal to BPTF / FALZ
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide from a region between residue 1350 and 1400 of human BPTF/ FALZ, NP_872579.2

  • Positive control
    • Whole cell lysate from Hela cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab72036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 324 kDa (predicted molecular weight: 324 kDa).

Several other bands.

IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • RelevanceBPTF (bromodomain and PHD domain transcription factor) is the largest subunit of the ATP-dependent chromatin-remodelling complex, NURF (nucleosome remodelling factor). NURF catalyses ATP-dependent nucleosome sliding and facilitates transcription. BPTF recognises histone H3 tails that are tri-methylated at K4, which marks the transcriptional start site of the vast majority of transcriptionally active genes. BPTF also exhibits some binding to H3 di-methylated at K4. BPTF plays a key role in the development of early mouse embryos, possibly through regulation of the Smad pathway of transcription factors. While BPTF is expressed in low levels in the adult brain and spinal cord, it is expressed in higher levels in the brain in neurodegenerative diseases. It is present in a subset of amyloid-containing plaques in the brains of patients suffering from Alzheimer's disease.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Bromodomain and PHD finger containing transcription factor antibody
    • Bromodomain PHD finger transcription factor antibody
    • FAC 1 antibody
    • fac1 antibody
    • FALZ antibody
    • Fetal Alz 50 clone 1 protein antibody
    • Fetal Alz 50 reactive clone 1 antibody
    • Fetal Alzheimer antigen antibody
    • Nucleosome remodeling factor antibody
    • Nucleosome remodeling factor subunit BPTF antibody
    • Nucleosome remodeling factor, large subunit antibody
    • NURF 301 antibody
    • NURF301 antibody
    see all

Anti-BPTF / FALZ antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BPTF / FALZ with ab72036 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BPTF / FALZ antibody (ab72036) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells. at 50 µg
    Lane 2 : Whole cell lysate from Hela cells. at 15 µg
    Lane 3 : Whole cell lysate from Hela cells. at 5 µg


    Predicted band size : 324 kDa
    Observed band size : 324 kDa
    Various other bands are detected from 117 to 460 kDa.
  • Immunoprecipitation/ Western Blot of FALZ / BPTF.
    Lane 1: ab72036 at 3µg/mg whole cell lysate for IP, and 0.1 µg/ml for subsequent WB.
    Lane 2: Control IgG.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection: Chemiluminescence with an exposure time of 1 second.
  • ICC/IF image of ab72036 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72036, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-BPTF / FALZ antibody (ab72036)

This product has been referenced in:
  • Mulder KW  et al. Diverse epigenetic strategies interact to control epidermal differentiation. Nat Cell Biol 14:753-63 (2012). ChIP . Read more (PubMed: 22729083) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"