Overview

  • Product name
  • Description
    Rabbit polyclonal to BPTF / FALZ
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1350-1400 of Human BPTF/ FALZ (NP_872579.2).

  • Positive control
    • Human Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Prostate Carcinoma and Testicular Seminoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab99399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Target

  • Function
    Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).
  • Sequence similarities
    Belongs to the PBTF family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 2 PHD-type zinc fingers.
  • Developmental stage
    Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).
  • Domain
    The second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine.
  • Post-translational
    modifications
    Phosphorylation enhances DNA-binding.
    Highly susceptible to proteolysis.
  • Cellular localization
    Cytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
  • Information by UniProt
  • Database links
  • Alternative names
    • BPTF antibody
    • BPTF_HUMAN antibody
    • Bromodomain and PHD finger containing transcription factor antibody
    • Bromodomain and PHD finger-containing transcription factor antibody
    • Bromodomain PHD finger transcription factor antibody
    • FAC 1 antibody
    • fac1 antibody
    • FALZ antibody
    • Fetal Alz 50 clone 1 protein antibody
    • Fetal Alz 50 reactive clone 1 antibody
    • Fetal Alz-50 clone 1 protein antibody
    • Fetal Alzheimer antigen antibody
    • Nucleosome remodeling factor antibody
    • Nucleosome remodeling factor subunit BPTF antibody
    • Nucleosome remodeling factor, large subunit antibody
    • Nucleosome-remodeling factor subunit BPTF antibody
    • NURF 301 antibody
    • NURF301 antibody
    see all

Anti-BPTF / FALZ antibody images

  • ab99399, at 1/250 dilution, staining BPTF / FALZ in formalin-fixed, paraffin-embedded Human breast carcinoma by Immunohistochemistry. Detection: DAB staining.
  • ab99399, at 1/250 dilution, staining BPTF / FALZ in formalin-fixed, paraffin-embedded Human colon carcinoma by Immunohistochemistry. Detection: DAB staining.

References for Anti-BPTF / FALZ antibody (ab99399)

ab99399 has not yet been referenced specifically in any publications.

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