Overview

  • Product nameAnti-Brachyury / Bry antibody
    See all Brachyury / Bry primary antibodies
  • Description
    Mouse monoclonal to Brachyury / Bry
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: ERSDHKEMME EPGDSQQPGY SQWGWLLPGT STLCPPANPH PQFGGALSLP STHSCDRYPT LRSHRSSPYP SPYAHRNNSP TYSDNSPACL SMLQSHDNW, corresponding to amino acids 222-321 of Human Brachyury/ Bry

Properties

Applications

Our Abpromise guarantee covers the use of ab57480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
  • Involvement in diseaseGenetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
    T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
  • Sequence similaritiesContains 1 T-box DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRAC_HUMAN antibody
    • Brachyury homolog antibody
    • Brachyury protein antibody
    • Bry antibody
    • MGC104817 antibody
    • Protein T antibody
    • SAVA antibody
    • T antibody
    • T brachyury homolog antibody
    • T brachyury transcription factor antibody
    • T Protein antibody
    • T, brachyury homolog (mouse) antibody
    • TFT antibody
    • Transcription factor T antibody
    see all

Anti-Brachyury / Bry antibody images

  • Anti-Brachyury / Bry antibody (ab57480) at 1 µg + A-549 cell lysate at 25 µg

    Predicted band size : 47 kDa

References for Anti-Brachyury / Bry antibody (ab57480)

This product has been referenced in:
  • Palena C  et al. Overexpression of the EMT driver brachyury in breast carcinomas: association with poor prognosis. J Natl Cancer Inst 106:N/A (2014). Human . Read more (PubMed: 24815864) »
  • Jezkova J  et al. Brachyury regulates proliferation of cancer cells via a p27Kip1-dependent pathway. Oncotarget 5:3813-22 (2014). IHC . Read more (PubMed: 25003467) »

See all 4 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Stem Cells)
Specification Stem Cells
Fixative Formalin
Permeabilization Yes - 0.1% Triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
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Verified customer

Submitted Jan 26 2012

Thank you for contacting us and reporting the problems your customer has been experiencing with anti-Brachyury / Bry antibody (ab57480).

We take product complaints very seriously, and investigate every product that we feel may not be perform...

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DISCOUNT CODE: xxxxxx Expiration date: 2012-04-02 I am very pleased to hear you would like to test ab57480 in IHC. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"