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Immunohistochemistry (Paraffin-embedded sections) - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
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Immunocytochemistry/ Immunofluorescence - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
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Western blot - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
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Product Name
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Brachyury / Bry antibody - Mesoderm Marker
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See all Brachyury / Bry antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Brachyury / Bry - Mesoderm Marker
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Brachyury.
(Peptide available as ab21992.)
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Reacts with
(species key)
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Hu, Ms
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Tested applications
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ICC/IF, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution (PMID 18172007).
IHC-P: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Stem Cells >> Lineage Markers >> Mesoderm
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Relevance
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This transcription factor is the earliest indicator of mesoderm formation. Used as a marker of mesodermal differentiation, Brachyury is expressed during gastrula and neurula stages in involuting mesoderm and in the notochord.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.50 - 0.60 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Brachyury / Bry antibody - Mesoderm Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Brachyury / Bry antibody - Mesoderm Marker images, references, products related to ab20680 and other tools.
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Brachyury / Bry antibody - Mesoderm Marker images:
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 Immunohistochemistry (Paraffin-embedded sections) - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
A proportion of nuclei from Day 3 Mouse EBs (derived from R1 cells) stained positive for Brachury using ab20680. In Day 8 EBs a vastly reduced proportion of nuclei stained positive (not shown). In day 10 EBs no nuclei stained Brachyury-positive. The IHC-P data for Brachyury in EBs corresponded with qRT-PCR expression data (not shown). Controls on Day 3 EBs in which primary or secondary antibody were omitted, undifferentiated ES cells and feeder cells (MEFs) all exhibited very little background staining.
Zinc was used for tissue fixation. Amanda Evans, University of Cambridge
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Immunocytochemistry/ Immunofluorescence - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
Anti-Brachyury antibody, ab20680, stained nuclei of human embryonic stem cells differentiated into mesendoderm. As would be expected, mesoderm cells express Brachyury whereas endoderm cells are negative.
Ludovic Valllier, University of Cambridge, UK
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Western blot - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
Brachyury / Bry antibody - Mesoderm Marker (ab20680) at 1 µg/ml + Murine ES Whole Cell Lysate Expressing Mesoderm Markers at 15 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
ab20680 recognizes a band of 53 kDa in Murine Embryonic Stem Cells differentiated to express mesoderm markers. This corresponds in size to the Brachyury protein which has a predicted molecular weight of 47 kDa. A band of 75 kDa has also been observed, although we are unsure as to the identity of this band.
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References for Brachyury / Bry antibody - Mesoderm Marker (ab20680)
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This product has been used in: (two most recent references)
Arpornmaeklong P et al. Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells. Stem Cells Dev 18:955-68 (2009). ICC/IF; Human. PubMed: 19327009
Vallier L et al. Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. PLoS One 4:e6082 (2009). ICC/IF; Human. PubMed: 19564924
See all 3 publication references for this product.
If you publish research using ab20680 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Brachyury / Bry
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