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Products:Stem Cells >> Lineage Markers >> Mesoderm
Overview
Product name
Anti-Brachyury / Bry antibody - Mesoderm Marker
See all Brachyury / Bry products (2) ...
Description
Rabbit polyclonal to Brachyury / Bry - Mesoderm Marker
Tested applications
ICC/IF, IHC-P, IHC-Fr, Flow Cyt, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Brachyury.
(Peptide available as ab21992.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent concentration. (PubMed: 21083428)
IHC-P: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Target
Function
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
Involvement in disease
Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
Sequence similarities
Contains 1 T-box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15178
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BRAC_HUMAN antibody
- Brachyury homolog antibody
- Brachyury protein antibody
see all
Database links
- Entrez Gene: 6862 Human
- Entrez Gene: 20997 Mouse
- Omim: 601397 Human
- SwissProt: O15178 Human
- SwissProt: P20293 Mouse
- Unigene: 389457 Human
- Unigene: 913 Mouse
Anti-Brachyury / Bry antibody - Mesoderm Marker images:
Immunohistochemistry (Paraffin-embedded sections) - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
A proportion of nuclei from Day 3 Mouse EBs (derived from R1 cells) stained positive for Brachury using ab20680. In Day 8 EBs a vastly reduced proportion of nuclei stained positive (not shown). In day 10 EBs no nuclei stained Brachyury-positive. The IHC-P data for Brachyury in EBs corresponded with qRT-PCR expression data (not shown). Controls on Day 3 EBs in which primary or secondary antibody were omitted, undifferentiated ES cells and feeder cells (MEFs) all exhibited very little background staining.
Zinc was used for tissue fixation.
Amanda Evans, University of Cambridge
Immunocytochemistry/ Immunofluorescence - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
Anti-Brachyury antibody, ab20680, stained nuclei of human embryonic stem cells differentiated into mesendoderm. As would be expected, mesoderm cells express Brachyury whereas endoderm cells are negative.
Ludovic Valllier, University of Cambridge, UK
Western blot - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
Anti-Brachyury / Bry antibody - Mesoderm Marker (ab20680) at 1 µg/ml + Murine ES Whole Cell Lysate Expressing Mesoderm Markers at 15 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
ab20680 recognizes a band of 53 kDa in Murine Embryonic Stem Cells differentiated to express mesoderm markers. This corresponds in size to the Brachyury protein which has a predicted molecular weight of 47 kDa. A band of 75 kDa has also been observed, although we are unsure as to the identity of this band.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Brachyury / Bry antibody - Mesoderm Marker (ab20680)
ab20680 staining Brachyury in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/2000 in horse serum) for 12 hours. A Streptavidin-conjugated Horse anti-rabbit monoclonal (1/250) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Jim Manavis
References for Anti-Brachyury / Bry antibody - Mesoderm Marker (ab20680)
This product has been referenced in:
- Shen Jet al. Classic chordoma coexisting with benign notochordal cell rest demonstrating different immunohistological expression patterns of brachyury and galectin-3. J Clin Neurosci 18:96-9 (2011). IHC-P; Human.Read more (PubMed: 20855213) »
- Wheadon Het al. Differential Hox expression in murine embryonic stem cell models of normal and malignant hematopoiesis. Stem Cells Dev 20:1465-76 (2011). Flow Cyt; Mouse.Read more (PubMed: 21083428) »
See all 8 publications for this product
Publishing research using ab20680? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"