Anti-Brachyury / Bry antibody - N-terminal (ab156611)

Overview

  • Product name
    Anti-Brachyury / Bry antibody - N-terminal
    See all Brachyury / Bry primary antibodies
  • Description
    Rabbit polyclonal to Brachyury / Bry - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Brachyury/ Bry aa 15-43 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: O15178

  • Positive control
    • WB: MDA-MB453 cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab156611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 47 kDa.

Target

  • Function
    Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
  • Involvement in disease
    Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
    T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
  • Sequence similarities
    Contains 1 T-box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRAC_HUMAN antibody
    • Brachyury homolog antibody
    • Brachyury protein antibody
    • Bry antibody
    • MGC104817 antibody
    • Protein T antibody
    • SAVA antibody
    • T antibody
    • T brachyury homolog antibody
    • T brachyury transcription factor antibody
    • T Protein antibody
    • T, brachyury homolog (mouse) antibody
    • TFT antibody
    • Transcription factor T antibody
    see all

Images

  • Anti-Brachyury / Bry antibody - N-terminal (ab156611) at 1/100 dilution + MDA-MB453 cell lysate at 35 µg

    Predicted band size: 47 kDa

  • Immunofluorescence of Hela cells labelling Brachyury/Bry with ab156611. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab156611 (1/25, 1 h at 37°C). An Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Brachyury/Bry immunoreactivity is localized to the nucleus.

References

ab156611 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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