• Product nameAnti-BRAF35 antibody
    See all BRAF35 primary antibodies
  • Description
    Rabbit polyclonal to BRAF35
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 25 and 75 of human BRAF35 (NP_006330.2).

  • Positive control
    • Whole cell lysate from HeLa cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7.0-8.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab72302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
IP Use at 2-5 µg/mg of lysate.


  • FunctionRequired for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters.
  • Tissue specificityUbiquitously expressed in adult tissues.
  • Sequence similaritiesContains 1 HMG box DNA-binding domain.
  • Cellular localizationNucleus. Chromosome. Localized to condensed chromosomes in mitosis in conjunction with BRCA2.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRAF25 antibody
    • BRAF35 antibody
    • BRCA2 associated factor 35 antibody
    • BRCA2-associated factor 35 antibody
    • High Mobility Group Protein 20B antibody
    • HM20B_HUMAN antibody
    • HMG box containing protein 20B antibody
    • HMG box-containing protein 20B antibody
    • HMG domain-containing protein 2 antibody
    • HMG domain-containing protein HMGX2 antibody
    • HMG20B antibody
    • SMARCE1-related protein antibody
    • SMARCE1r antibody
    • sox like antibody
    • Sox like transcriptional factor antibody
    • Sox-like transcriptional factor antibody
    • SOXL antibody
    • Structural DNA binding protein BRAF35 antibody
    • Structural DNA-binding protein BRAF35 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related antibody
    see all

Anti-BRAF35 antibody images

  • All lanes : Anti-BRAF35 antibody (ab72302) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 28 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human BRAF35 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72302 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

References for Anti-BRAF35 antibody (ab72302)

ab72302 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount 20 µg
Specification lymphoblastoid cell line
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 13 2011