Overview

  • Product name
  • Description
    Rabbit polyclonal to BRAP
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 BRAP.

    (Peptide available as ab88155.)

  • Positive control
    • This antibody gave a positive signal in MCF7 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
ICC/IF 1/200. Use with paraformaldehyde fixed cells.

Target

  • Relevance
    BRCA1 Associated Protein, BRAP2 (Impedes mitogenic signal propogation; IMP) is mainly cytoplasmic and binds to the NLS motifs of BRCA1 with similar specificity to that of importin alpha. BRAP2 may function as a cytoplasmic retention protein and play a role in regulating transport of nuclear proteins. More recently, the protein has been shown to impede signal transmission from Raf to MEK.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • 3010002G07Rik antibody
    • BRAP2 antibody
    • BRCA1 associated protein antibody
    • EC 6.3.2. antibody
    • Galectin 2 binding protein antibody
    • IMP antibody
    • Impedes mitogenic signal propagation antibody
    • Renal carcinoma antigen NY REN 63 antibody
    • RING finger protein 52 antibody
    • RNF52 antibody
    • zgc:92894 antibody
    see all

Images

  • Anti-BRAP antibody (ab77721) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa,34 kDa. We are unsure as to the identity of these extra bands.
  • ab77721 (1/200) staining BRAP in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

References

ab77721 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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