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Synthetic peptide corresponding to Human BRCA2.
Our Abpromise guarantee covers the use of ab9143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 12915460|
|IP||Use a concentration of 1 - 4 µg/ml.|
|WB||1/1000 - 1/10000. Detects a band of approximately 380 kDa (predicted molecular weight: 380 kDa).|
ab9143 immunoprecipitating BRCA2 at 6 µg per reaction.
Lane 1: ab9143 in HEK2936 cell lysate.
Lane 2: Rabbit polyclonal antibody (ab123491) used instead of ab9143 in 293T cell lysate
Lane 3 (control): Secondary antibody only control
For blotting, ab9143 immunoprecipitated BRAC2 at 1 µg/ml. Detected by Chemiluminescence.