Overview

  • Product nameAnti-BRCC36 antibody
    See all BRCC36 primary antibodies
  • Description
    Rabbit polyclonal to BRCC36
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Pig, Rhesus monkey, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 266 -316 of Human BRCC36 (P46736).

  • Positive control
    • HeLa, 293T and mouse NIH3T3 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 36 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMetalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors.
  • Involvement in diseaseA chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA.
  • Sequence similaritiesBelongs to the peptidase M67A family. BRCC36 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm (PubMed:20656690).
  • Information by UniProt
  • Database links
  • Alternative names
    • BRCA1-A complex subunit BRCC36 antibody
    • BRCA1/BRCA2 containing complex subunit 3 antibody
    • BRCA1/BRCA2 containing complex subunit 36 antibody
    • BRCA1/BRCA2-containing complex subunit 3 antibody
    • BRCA1/BRCA2-containing complex subunit 36 antibody
    • Brcc3 antibody
    • BRCC3_HUMAN antibody
    • BRCC36 antibody
    • BRISC complex subunit BRCC36 antibody
    • C6.1A antibody
    • Chromosome X open reading frame 53 antibody
    • CXorf53 antibody
    • Lys-63-specific deubiquitinase BRCC36 antibody
    • OTTHUMP00000061450 antibody
    see all

Anti-BRCC36 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRCC36 with ab115172 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BRCC36 antibody (ab115172) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 cell lysate at 50 µg


    Predicted band size : 36 kDa


    Exposure time : 30 seconds
  • Immunoprecipitation of BRCC36 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1-Immunoprecipitation with an anti-BRCC36 antibody that recognizes an upstream epitope; Lane 2-Immunoprecipitation with anti-BRCC36 antibody (ab115172); Lane 3-Immunoprecipitation with IgG control. Anti-BRCC36 antibody (ab115172) used at 0.4 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.

References for Anti-BRCC36 antibody (ab115172)

This product has been referenced in:
  • Jamsai D  et al. Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse. PLoS One 8:e56955 (2013). WB ; Mouse . Read more (PubMed: 23451117) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"