Overview

  • Product nameAnti-BRCC36 antibody
    See all BRCC36 primary antibodies
  • Description
    Rabbit polyclonal to BRCC36
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Raised against an 18 amino acid peptide from near the amino terminus of human BRCC36.

  • Positive control
    • MCF7 nuclear lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionMetalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors.
  • Involvement in diseaseA chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA.
  • Sequence similaritiesBelongs to the peptidase M67A family. BRCC36 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm (PubMed:20656690).
  • Information by UniProt
  • Database links
  • Alternative names
    • BRCA1-A complex subunit BRCC36 antibody
    • BRCA1/BRCA2 containing complex subunit 3 antibody
    • BRCA1/BRCA2 containing complex subunit 36 antibody
    • BRCA1/BRCA2-containing complex subunit 3 antibody
    • BRCA1/BRCA2-containing complex subunit 36 antibody
    • Brcc3 antibody
    • BRCC3_HUMAN antibody
    • BRCC36 antibody
    • BRISC complex subunit BRCC36 antibody
    • C6.1A antibody
    • Chromosome X open reading frame 53 antibody
    • CXorf53 antibody
    • Lys-63-specific deubiquitinase BRCC36 antibody
    • OTTHUMP00000061450 antibody
    see all

Anti-BRCC36 antibody images

  • Lane 1 : Anti-BRCC36 antibody (ab62075) at 0.5 µg/ml
    Lane 2 : Anti-BRCC36 antibody (ab62075) at 1 µg/ml
    Lane 3 : Anti-BRCC36 antibody (ab62075) at 2 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    anti-rabbit IgG

    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab62075 at 2.5 µg/ml staining BRCC36 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Immunofluorescence of BRCC36 in Human Brain cells using ab62075 at 20 ug/ml.

References for Anti-BRCC36 antibody (ab62075)

ab62075 has not yet been referenced specifically in any publications.

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