Overview

  • Product name
  • Description
    Rabbit polyclonal to BRCC45
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BRCC45 aa 340-370 (C terminal). The exact sequence is proprietary.
    Database link: Q9NXR7-1

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab192315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 44 kDa.

Target

  • Function
    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.
  • Tissue specificity
    Expressed in all cell lines examined. Highly expressed in placenta.
  • Sequence similarities
    Belongs to the BRE family.
  • Domain
    Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6030405P19Rik antibody
    • AI429776 antibody
    • B830038C02Rik antibody
    • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
    • brain and reproductive organ expressed protein antibody
    • Brain and reproductive organ-expressed protein antibody
    • BRCA1 A complex subunit BRE antibody
    • BRCA1-A complex subunit BRE antibody
    • BRCA1/BRCA2 containing complex subunit 4 antibody
    • BRCA1/BRCA2 containing complex subunit 45 antibody
    • BRCA1/BRCA2-containing complex subunit 45 antibody
    • BRCC4 antibody
    • BRCC45 antibody
    • bre antibody
    • BRE_HUMAN antibody
    see all

Images

  • Anti-BRCC45 antibody (ab192315) at 1/1000 dilution + HeLa cell lysate at 35 µg

    Secondary
    Goat anti-rabbit IgG H&L (HRP) at 1/5000 dilution

    Predicted band size : 44 kDa

References

ab192315 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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