Recombinant
RabMAb

Anti-BRCC45 antibody [EPR11858] (ab177960)

Overview

  • Product name
    Anti-BRCC45 antibody [EPR11858]
    See all BRCC45 primary antibodies
  • Description
    Rabbit monoclonal [EPR11858] to BRCC45
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BRCC45 aa 1-100. The exact sequence is proprietary.
    Database link: Q9NXR7

  • Positive control
    • fetal brain, Human placenta, HeLa, and Raji lysates; Human lung adenocarcinoma tissue; Human pancreas tissue; MCF-7 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
ICC/IF 1/50 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.
    • Tissue specificity
      Expressed in all cell lines examined. Highly expressed in placenta.
    • Sequence similarities
      Belongs to the BRE family.
    • Domain
      Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.
    • Cellular localization
      Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6030405P19Rik antibody
      • AI429776 antibody
      • B830038C02Rik antibody
      • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
      • brain and reproductive organ expressed protein antibody
      • Brain and reproductive organ-expressed protein antibody
      • BRCA1 A complex subunit BRE antibody
      • BRCA1-A complex subunit BRE antibody
      • BRCA1/BRCA2 containing complex subunit 4 antibody
      • BRCA1/BRCA2 containing complex subunit 45 antibody
      • BRCA1/BRCA2-containing complex subunit 45 antibody
      • BRCC4 antibody
      • BRCC45 antibody
      • bre antibody
      • BRE_HUMAN antibody
      see all

    Images

    • Immunohistochemical analysis of Human pancreas tissue labeling BRCC45 using ab177960 at 1/50 dilution.

    • Immunohistochemical analysis of Human lung adenocarcinoma tissue labeling BRCC45 using ab177960 at 1/50 dilution.

    • Immunofluorescence analysis of MCF-7 cells labeling BRCC45 using ab177960 at 1/50 dilution.

    • All lanes : Anti-BRCC45 antibody [EPR11858] (ab177960) at 1/1000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : Human placenta lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 44 kDa

    References

    ab177960 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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