Overview

  • Product nameAnti-BRD1 antibody
    See all BRD1 primary antibodies
  • Description
    Rabbit polyclonal to BRD1
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human BRD1.

    (Peptide available as ab71876.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: TE 671 Raji

Properties

Applications

Our Abpromise guarantee covers the use of ab71877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 137 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.
    • Tissue specificityHighly expressed in testis.
    • Sequence similaritiesContains 1 bromo domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 PWWP domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BR140-like protein antibody
      • BRD1 antibody
      • BRD1_HUMAN antibody
      • BRL antibody
      • Bromodomain and PHD finger-containing protein 2 antibody
      • Bromodomain containing 1 antibody
      • Bromodomain-containing protein 1 antibody
      • BRPF1 antibody
      • BRPF2 antibody
      • DKFZp686F0325 antibody
      see all

    Anti-BRD1 antibody images

    • All lanes : Anti-BRD1 antibody (ab71877) at 1 µg/ml

      Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
      Lane 2 : Raji whole cell lysate (ab30124) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 137 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab71877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71877, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
    • ab71877 staining BRD1 in mouse testis tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocking (5 minutes of peroxidase block and 10 minutes of protein block) for 15 minutes at 20°C was performed. The primary antibody was diluted 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.

      The image indicated a specific nuclear staining and very low background. Arrows indicate some of the positive cells.

      See Abreview

    • All lanes : Anti-BRD1 antibody (ab71877) at 1 µg/ml

      Lane 1 : Lysates prepared from Mouse testis tissue.
      Lane 2 : Lysates prepared from Mouse Lung tissue.
      Lane 3 : Lysates prepared from Mouse Kidney tissue.
      Lane 4 : Lysates prepared from GC-1 cell line.

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated pig polyclonal to rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 137 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 34 kDa,92 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 7 minutes

      This image is a courtesy of Antibody Solutions Ltd

      See Abreview

    References for Anti-BRD1 antibody (ab71877)

    ab71877 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (testis)
    Specification testis
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 14 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Ms Testis, Ms Lung, Ms Kidney, GC-1)
    Loading amount 20 µg
    Specification Ms Testis, Ms Lung, Ms Kidney, GC-1
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"