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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab111078 was affinity purified using an epitope specific to BRD2 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
1/100 - 1/500. Formaldehyde-Fixed.
Permeabilization with Triton-X 100 is recommended.
Function May play a role in spermatogenesis or folliculogenesis.
Sequence similarities Contains 2 bromo domains. Contains 1 ET domain.
Domain One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
Cellular localization Nucleus.
Information by UniProt
BRD 2 antibody
Anti-BRD2 antibody images
Immunocytochemistry/ Immunofluorescence - BRD2 antibody (ab111078)
ab111078 at 1/250 dilution staining BRD2 in formaldehyde-fixed asynchronous HeLa cells by cytochemistry.
Detection: Red fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BRD2 antibody (ab111078)
ab111078 at 1/250 dilution staining BRD2 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
References for Anti-BRD2 antibody (ab111078)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"