Overview

  • Product nameAnti-BRD2 antibody
    See all BRD2 primary antibodies
  • Description
    Rabbit polyclonal to BRD2
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 675 and 725 of Human BRD2 (NP_005095.1).

  • Positive control
    • Human Breast Carcinoma, Colon Carcinoma and Prostate Carcinoma tissues; HeLa Cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab111078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
ICC/IF 1/100 - 1/500. Formaldehyde-Fixed. Permeabilization with Triton-X 100 is recommended.

Target

  • FunctionMay play a role in spermatogenesis or folliculogenesis.
  • Sequence similaritiesContains 2 bromo domains.
    Contains 1 ET domain.
  • DomainOne bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRD 2 antibody
    • Brd2 antibody
    • BRD2_HUMAN antibody
    • Bromodomain containing 2 antibody
    • Bromodomain-containing protein 2 antibody
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Anti-BRD2 antibody images

  • ab111078 at 1/250 dilution staining BRD2 in formaldehyde-fixed asynchronous HeLa cells by cytochemistry.
    Detection: Red fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 used at a dilution of 1/100.
  • ab111078 at 1/250 dilution staining BRD2 in Human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
    Detection: DAB staining.

References for Anti-BRD2 antibody (ab111078)

ab111078 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (MEFs)
Loading amount 15 µg
Specification MEFs
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Submitted Jan 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"