Overview

  • Product nameAnti-BRD2 antibody
    See all BRD2 primary antibodies
  • Description
    Goat polyclonal to BRD2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of human Bromodomain Containing 2 (BRD 2) between the putative nuclear localization signal and the ET domain.

  • Positive control
    • HeLa cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/40000. Predicted molecular weight: 110 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 22215678

Target

Anti-BRD2 antibody images



  • Predicted band size : 110 kDa


    Sample: Hela cell total RIPA extract (10µg) Antibody: Affinity purified Goat anti-BRD 2 (ab3718) (diluted 1 / 20,000) CIP: Calf Intestinal Phosphatase Detection: ECL exposed for 1 minute.

References for Anti-BRD2 antibody (ab3718)

This product has been referenced in:
  • Dhar S  et al. Insights into role of bromodomain, testis-specific (Brdt) in acetylated histone H4-dependent chromatin remodeling in mammalian spermiogenesis. J Biol Chem 287:6387-405 (2012). ICC/IF ; Rat . Read more (PubMed: 22215678) »
  • Draker R  et al. A combination of H2A.Z and H4 acetylation recruits Brd2 to chromatin during transcriptional activation. PLoS Genet 8:e1003047 (2012). WB . Read more (PubMed: 23144632) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic fibroblasts)
Loading amount 15 µg
Specification embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 22 2013

Thank you for contacting us.

Yes, ab3718 can recognise both the phosphorylated and unphosphorylated BRD2.

For ab113117, we recommend to use 1ug/ml (1:1000) in WB.

I hope this information is helpful to you. Please do not h...

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Thank you for contacting us.

The target protein got a number of phosphorserine and phosphothreonine residues which can increase the molecular weight of the protein.

I hope this information is helpful to you. Please do not hesitate ...

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Thank you for your enquiry. I have requested that the lab performs an alignment of the immunogen sequences with the rat homologs. Details of the degree of similarity is below: BRD2(rat) - GeneID: 294276 (NP_997660) The peptide immunogen w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"