Overview

  • Product nameAnti-BRD2 antibody
    See all BRD2 primary antibodies
  • Description
    Rabbit polyclonal to BRD2
  • Tested applicationsSuitable for: ELISA, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to sequence from within amino acids 100-200 of Human BRD2.

  • Positive control
    • A375 cell lysate, Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab37633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionMay play a role in spermatogenesis or folliculogenesis.
    • Sequence similaritiesContains 2 bromo domains.
      Contains 1 ET domain.
    • DomainOne bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BRD 2 antibody
      • Brd2 antibody
      • BRD2_HUMAN antibody
      • Bromodomain containing 2 antibody
      • Bromodomain-containing protein 2 antibody
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    Anti-BRD2 antibody images

    References for Anti-BRD2 antibody (ab37633)

    This product has been referenced in:
    • Thompson CM  et al. Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems. BMC Biotechnol 15:31 (2015). WB . Read more (PubMed: 25981500) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Compact polymer kit used, no additional blocking needed as blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate based buffer, pH 6.0
    Sample Human Tissue sections (Metastatic melanoma)
    Specification Metastatic melanoma
    Permeabilization Yes - Surfactant in washing reagent
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 24 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Fruit fly (Drosophila melanogaster) Cell (embryo)
    Specification embryo
    Fixative Formaldehyde
    Permeabilization Yes - 0.1% Triton X-100
    Username

    Mr. Esteban Contreras Sepúlveda

    Verified customer

    Submitted Feb 15 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"