• Product nameAnti-BRD3 antibody
    See all BRD3 primary antibodies
  • Description
    Mouse monoclonal to BRD3
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 418-556 of Human BRD3



Our Abpromise guarantee covers the use of ab56342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • Tissue specificityUbiquitous.
  • Involvement in diseaseNote=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
  • Sequence similaritiesContains 2 bromo domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRD 3 antibody
    • Brd3 antibody
    • BRD3_HUMAN antibody
    • Bromodomain containing 3 antibody
    • Bromodomain containing protein 3 antibody
    • Bromodomain-containing protein 3 antibody
    • KIAA0043 antibody
    • Open reading frame X antibody
    • ORFX antibody
    • RING3 like gene antibody
    • RING3-like protein antibody
    • RING3L antibody
    see all

Anti-BRD3 antibody images

  • BRD3 antibody (ab56342) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
  • BRD3 antibody (ab56342) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab56342 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56342, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-BRD3 antibody (ab56342)

This product has been referenced in:
  • Boi M  et al. Therapeutic efficacy of the bromodomain inhibitor OTX015/MK-8628 in ALK-positive anaplastic large cell lymphoma: an alternative modality to overcome resistant phenotypes. Oncotarget 7:79637-79653 (2016). WB . Read more (PubMed: 27793034) »
  • Thompson CM  et al. Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems. BMC Biotechnol 15:31 (2015). WB . Read more (PubMed: 25981500) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ventana CC2 (citrate pH6.0)
Permeabilization No
Specification Testis
Blocking step Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jun 20 2017

Thank you for your inquiry.

We do not specifically test this antibody for it's cross-reactivity to other Brd family members, however based on a BLAST of the immunogen (AAH32124, 418 a.a. ˜ 556 a.a), we would not expect it to cross-react...

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