Overview

  • Product name
  • Description
    Mouse monoclonal to BRD3
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 418-556 of Human BRD3

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab56342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Note=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
  • Sequence similarities
    Contains 2 bromo domains.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRD 3 antibody
    • Brd3 antibody
    • BRD3_HUMAN antibody
    • Bromodomain containing 3 antibody
    • Bromodomain containing protein 3 antibody
    • Bromodomain-containing protein 3 antibody
    • KIAA0043 antibody
    • Open reading frame X antibody
    • ORFX antibody
    • RING3 like gene antibody
    • RING3-like protein antibody
    • RING3L antibody
    see all

Anti-BRD3 antibody images

  • BRD3 antibody (ab56342) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
  • BRD3 antibody (ab56342) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab56342 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56342, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-BRD3 antibody (ab56342)

This product has been referenced in:
  • Vázquez R  et al. The bromodomain inhibitor OTX015 (MK-8628) exerts anti-tumor activity in triple-negative breast cancer models as single agent and in combination with everolimus. Oncotarget 8:7598-7613 (2017). WB . Read more (PubMed: 27935867) »
  • Tanaka M  et al. Design and characterization of bivalent BET inhibitors. Nat Chem Biol 12:1089-1096 (2016). WB . Read more (PubMed: 27775715) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC2 (citrate pH6.0)
Permeabilization
No
Specification
Testis
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 20 2017

Thank you for your inquiry.


We do not specifically test this antibody for it's cross-reactivity to other Brd family members, however based on a BLAST of the immunogen (AAH32124, 418 a.a. ˜ 556 a.a), we would not expect it to cross-react...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up