Overview

  • Product nameAnti-BRD3 antibody
    See all BRD3 primary antibodies
  • Description
    Rabbit polyclonal to BRD3
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human BRD3 conjugated to KLH

  • Positive control
    • Mouse brain tissue and Saos-2 cell lysates and human breast carcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/25 - 1/100.
WB 1/100 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).

Target

  • Tissue specificityUbiquitous.
  • Involvement in diseaseNote=A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein.
  • Sequence similaritiesContains 2 bromo domains.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRD 3 antibody
    • Brd3 antibody
    • BRD3_HUMAN antibody
    • Bromodomain containing 3 antibody
    • Bromodomain containing protein 3 antibody
    • Bromodomain-containing protein 3 antibody
    • KIAA0043 antibody
    • Open reading frame X antibody
    • ORFX antibody
    • RING3 like gene antibody
    • RING3-like protein antibody
    • RING3L antibody
    see all

Anti-BRD3 antibody images

  • All lanes : Anti-BRD3 antibody (ab71815) at 1/1000 dilution

    Lane 1 : mouse brain tissue lysate
    Lane 2 : Saos-2 cell lysate

    Lysates/proteins at 12.5 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling BRD3 C-terminal with ab71815 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

References for Anti-BRD3 antibody (ab71815)

ab71815 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (Embryo)
Specification Embryo
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Username

Mr. Esteban Contreras Sepúlveda

Verified customer

Submitted Feb 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"